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Total RNA was isolated from the MC3T3-E1 cells using the rneasy mini kit (qiagen, Inc., Texas, USA). cDNA was synthesized using the superscript vilo cdna synthese kit (Invitrogen, USA) following the.
Collectively, these results support a convergent molecular abnormality in CP and ASD. This study was approved by the Women’s and Children’s health network (wchn) human research Ethics Committee.
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“FIV” is typically used to refer to lentiviruses arising from domestic cats; when more than one feline species of lentivirus is being discussed, the standard nomenclature for designation of strains originating from different species is to append genus and species identifiers for the feline species as a subscript to FIV; for example, domestic cat (Felis cattus) FIV becomes FIVfca, African lion (Panthera leo) FIV is.
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Next, RNA was DNaseI (ThermoFisher) treated and the mRNA was purified and fragmented. First strand cDNA synthesis was performed using SuperScript II (ThermoFisher) followed by second strand cDNA.